Table 1 Fold change in the detection of four viruses by total nucleic acid extraction methods compared to QIAamp® MinElute® Virus Spin kit

From: Selection and evaluation of an efficient method for the recovery of viral nucleic acids from complex biologicals

Sample extraction method

Detection of EBV

Detection of FeLV

Detection of Reo3

Detection of RSV

Invitrogen PureLink™a

−1.55

1.68

7.89

2.31

QIAGEN Circulating Nucleic Acida

−1.27

−2.50

−1.72

−1.32

Clonetech Nucleospin®a

−15.46

−62.25

−1.18

−8.00

Invitrogen Dynabeads®b

−1.02

1.22

−1.01

1.23

Perkin Elmer Chemagic™b

−1.49

−2.66

−5.54

−3.39

Phenol:Chloroformc

−27.86

−30.06

−7.52

−8.75

Acid phenol:chloroformc

−7.94

−3.36

9.58

1.69

  1. Sample extraction methods were assessed for their efficient extraction of four representative viruses. Silica membrane column- (a), magnetic bead- (b), and precipitation (c)-based extractions were included
Back to article page