Table 2 Sequencing results comparing WGA and double-stranded DNA synthesis

From: Selection and evaluation of an efficient method for the recovery of viral nucleic acids from complex biologicals

Virus

Double-stranded DNA synthesis

Fold change in detection

First strand → WGA

First strand → second strand

Total reads

342 470 258

239 327 708

 

EBV

3.73% (12 770 076)

0.44% (1 049 436)

0.12

RSV

0.00038% (1287)

0.0094% (22 579)

25

FeLV

0.00030% (1032)

0.030% (71 024)

100

Reo3

0.015% (52 114)

0.31% (752 834)

21

  1. The introduction of any bias in the viral population after WGA and/or double-stranded DNA synthesis was assessed using high-throughput sequencing. Extracted nucleic acid was converted to double-stranded DNA by first carrying out first-strand synthesis. Following first-strand synthesis the two techniques were compared for conversion to double-stranded DNA
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