Table 3 Comparison of the selected sample preparation procedure to extraction using the QIAamp® MinElute® Virus Spin kit followed by WGA

From: Selection and evaluation of an efficient method for the recovery of viral nucleic acids from complex biologicals

Virus

MinElute® + WGA (average of n = 3 ± standard deviation)

Optimized procedure (average of n = 4 ± standard deviation)

Fold change in detection

EBV

0.36 ± 0.15%

0.34% ± 0.10

0.94

RSV

0.0012 ± 0.0007%

0.0076% ± 0.0021

6.3

FeLV

0.0022 ± 0.0014%

0.036% ± 0.008

16

Reo3

0.000045 ± 0.000038%

0.38% ± 0.14

8400

  1. The selected procedure, which involved the dual extraction strategy using both Invitrogen PureLink™ Virus RNA/DNA kit and the Wako DNA Extractor® kit with the RNase A digestion and the double-stranded DNA synthesis, was compared against the QIAGEN QIAamp® MinElute® Virus Spin kit followed by whole-genome amplification (WGA). Reported values are an average of at least three replicates with their standard deviation
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