Fig. 2
Empirical and model predicted number of IFN-γ secreting CD4+ T cells over time for a low dose group (0.1–1 µg H56 + IC31), b middle dose group (5 µg H56 + IC31), c high dose group (15 µg H56 + IC31) and human dose group (50 µg H56 + IC31). Grey points correspond to number of IFN-γ secreting CD4+ T cells measured over time by ELISPOT assay (in mouse splenocytes A, B and C and PBMC in d) after receiving two vaccinations of H56 + IC31 (day 0 and 15, for mice, day 0 and 56 for humans). Median responses over time are marked by a blue triangle, the 75th percentile responses by an orange triangle and the 25th percentile responses by a purple triangle. The model prediction (total cells) fitted to the data in the fitting framework (parameters in Table 1) is plotted against the median data (blue line). The orange and purple dashed lines are the model prediction (total cells) of the 75th and 25th percentiles of the data, a result of the variation in the estimated parameters (standard deviation fixed to 0.5 for all parameters (Table 1))
