Fig. 1 | npj Vaccines

Fig. 1

From: A recombinant conjugated pneumococcal vaccine that protects against murine infections with a similar efficacy to Prevnar-13

Fig. 1

Analysis of recombinant proteins from glycoprotein expression strains. a-c Immunoblot analysis of decreasing concentrations of recombinant glycoproteins from galE+ and galE- E. coli strains. Fluorescent intensity of the signal from the glycosylated protein bands (green channel) was measured by densitometry using the LI-COR odyssey fluorescent imaging system and are displayed as mean ± SEM from three replicate experiments. Red channel: mouse anti-His IgG; Green channel: rabbit anti-Type 4 antiserum. d-h Anti-capsular polysaccharide antibody levels in antiserum measured from mice (n = 8) vaccinated with recombinant glycoproteins (closed circles) or cognate unglycosylated antigens (open squares). Antiserum from Prevnar-13 (closed circles) and PBS vaccinated (open squares) animals were included as controls. Results displayed as mean ± SEM from technical replicates

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