Fig. 5

Gene set enrichment analysis (GSEA) of upregulated or downregulated gene sets curated from related vaccination mircoarray studies. a An illustration of related microarray studies (first row), with the vaccine (second row) and the corresponding condition/variable (third row). b, c Peak normalised gene set enrichment scores for the (t statistic) ranked gene lists for each group is displayed as a bar chart. Each bar chart corresponds to each vaccination condition, needle and syringe intradermal (ID) 50 J/m2 with vaccine protein, Nanopatch™ 3500 J/m2 blank, Nanopatch™ 3500 J/m2 with vaccine protein, Nanopatch™ 3500 J/m2 with vaccine protein and QS-21, and Nanopatch™ 11,200 J/m2 with vaccine protein compared with unimmunised control. The b upregulated and c downregulated gene sets are plotted against normalised enrichment score with significance represented by black horizontal bars (nominal p value < 0.05 and FDR q-value < 0.25). All groups n = 3, except naive samples n = 6