Fig. 1
From: An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses
ZIKV NS4B-C100S mutant is attenuated in AG129 mice. a Amino acid alignments for WNV and ZIKV in the conserved N-terminal region (i) and central hydrophobic domain (ii) of NS4B. b, c Six-week-old AG129 mice (n = 4 or 5) were immunized i.p. with 10 or 2 × 104 FFU WT and mutant viruses and PBS (mock), respectively. The immunized mice were monitored for weight loss (b) and survival (c). Weight loss is indicated by percentage, using the weight on the day before immunization to define 100%. d Six-week-old AG129 mice (n = 4 or 5) were infected with either 10 FFU of WT ZIKV, or a low (L, 2 × 104 FFU) or high (H, 1 × 105 FFU) dose of NS4B-C100S. Viremia were measured on day 3 (n = 9 to 15). Data are presented as means ± SEM. **P < 0.01 compared with the WT group (unpaired t-test).
