Fig. 2
From: An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses
ZIKV NS4B-C100S mutant is attenuated in AB6 mice. a, b Six-week-old AB6 mice were immunized i.p. with 2 × 104 FFU WT or ZIKV NS4B-C100S. The immunized mice were monitored for weight loss (a) and survival (b). Weight loss is indicated by percentage, using the weight on the day before immunization to define 100%. n = 10 and n = 8 for WT and NS4B-C100S-infected mice, respectively. **P < 0.01 compared with WT group (log-rank test). c–h Six-week-old AB6 mice were immunized i.p. with 2 × 104 FFU WT (red dot), ZIKV NS4B-C100S (blue dot), or PBS (mock, open triangle). c, d Viremia was determined by using Q-PCR and FFA at day 2 and day 6 post infection (pi). e–h Viral loads in the kidney, liver, brain, and testis were determined by Q-PCR assay. Data are presented as means ± SEM, n = 5–10 and are representative two similar experiments. **P < 0.01 or *P < 0.05 compared with the WT group (unpaired t-test).
