Fig. 3: Peptide-specific responses of IFNγ secreting PBMCs from patient groups.
From: Identification of antigens presented by MHC for vaccines against tuberculosis
ELISpots of PBMCs from 14 BCG-vaccinated individuals HLA-matched (n = 5), BCG-vaccinated individuals non-HLA matched (n = 8), latently infected individuals HLA-matched (n = 7), latently infected individuals non-HLA matched (n = 10 in (a, b, c, d, e); n = 9 in (f) and tuberculosis active patients (n = 5 in (a, b, c, d, e); n = 4 in (f) were used. Cells were stimulated with 2μg/ml of individual or pooled peptides or 20μg/ml PPD, or left unstimulated, with 3 × 105 cells per well in duplicates. Cells were stimulated with (a) fbpA44–51 FSRPGLPV, (b) BCG_3870c4–12 LAASLLSRV, (c) fas57–65 GIETELATL, (d) iniC191–200 QIGGLVGGSV, (e) groL261–75 LEDPYEKIGAELVKE and (f) a pool of peptides containing these five sequences and mmpL12396–403 AGCTLLIR and atpA333–340 KANDISAY (a peptide identified with a Peaks score of 14.54 hence excluded from the analysis). Each dot represents the results from each peptide. The error bars show the mean and standard errors of the mean. Statistical analysis performed with Two-tailed Mann–Whitney U test, and statistically significant differences between groups are represented as *p < 0.05, **p < 0.01.
