Table 1 Description of the samples.

From: Identification of antigens presented by MHC for vaccines against tuberculosis

 

Exp 1

Exp 2

Exp 3

Exp 4

Number of cells per sample

2.5 × 108

2.5 × 108

5 × 107

5 × 108

Rate of infection (%)

30

30

40

21

Culture flasks

T1000

T175

T175

T175

Cytokine pre-treatment

No

No

Yes

Yes

Heat Killed BCG

No

No

Yes

Yes

Immunoprecipitation

MHC-I

MHC-I and -II

MHC-I and -II

MHC-I and -II

Number of samples

3

3

6

6

Number of total replicates

6

9

12

22

Name of samples

CONTROL (A, B)

CONTROL (A, B)a

NOCYT NOINF

NOCYT NOINF (A, B)

(replicates A/B)

DAY 1 (A, B)

DAY 1 (A, B)a

NOCYT HKBCG

NOCYT HKBCG (A, B)

 

DAY 7 (A, B)

DAY 7 (A, B)a

NOCYT LIVEBCG

NOCYT LIVEBCG (A, B)

   

CYT NOINF

CYT NOINF (A, B)

   

CYT HKBCG

CYT HKBCG (A,B)a

   

CYT LIVEBCG

CYT LIVEBCG (A, B)a

  1. aSamples with two replicates for MHC-I and one replicate for MHC-II