Fig. 2: Human HA-specific CD4 T-cell responses post vaccination. | npj Vaccines

Fig. 2: Human HA-specific CD4 T-cell responses post vaccination.

From: Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans

Fig. 2

CD4-enriched cells from the vaccinated subjects were stimulated with overlapping pools of peptides from H1, H3 and HA-B proteins and evaluated for IFN-γ production using cytokine EliSpot assays. The responses for the 3 groups Fluzone (blue), Flucelvax (gold) or Flublok (red) are indicated. In a, the CD4 T cells expanded between day 0 and day 14 for each HA peptide pool tested were summed. The data are represented as the change in the response between day 0 and day 14 (D14–D0) with the median response indicated by a black line. In b, the expansion of CD4 T cells for each HA peptide set is shown for the HA protein that is indicated above each panel. H1 is shown on the left, H3 in the middle and HA-B on the right. The top panels illustrate the response of each individual, represented as a single dot with the spread in reactivity of the population and the median value indicated by a black line. The bottom bar graphs indicate the average response of the individuals shown in the top panel and the 95% confidence interval of each group for each HA. The p values shown were calculated by the Wilcoxon rank sum test.

Back to article page