Fig. 5: Polyfunctional CD4 T-cell response to vaccination with three vaccine platforms. | npj Vaccines

Fig. 5: Polyfunctional CD4 T-cell response to vaccination with three vaccine platforms.

From: Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans

Fig. 5

PBMC from subjects vaccinated with Fluzone (blue), Flucelvax (gold) or Flublok (red) were stimulated in vitro with pools of peptides derived from H1 (left) or H3 (right) for 16 h. Brefeldin A and monensin were added for the last 8 h of culture to block cytokine secretion. H1- or H3-specific CD4 T cells were identified based on expression of CD69 + and the intracellular cytokines IL-2 and IFN-γ. Shown is quantification of cells producing both cytokines, IFN-γ + IL2 + (left), IFN-γ + IL2- (middle) and IFN-γ-IL2 + (right), using the gating scheme shown in Supplementary Fig. 5. The data are represented as the gain in the number in antigen-reactive, cytokine-positive cells between day 0 and day 7. The average response is indicated by the grey bar and the individual responses are indicated by the scatter. The p values shown were calculated by the Wilcoxon rank sum test.

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