Fig. 7: Depletion of IL-4 increases efficacy of anti-fentanyl vaccines.
From: Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders
Balb/c mice were immunized with either F-sKLH or F-CRM, alone or in combination with αIL-4, and then challenged s.c. with 0.05 mg/kg fentanyl. Fentanyl-specific (a) IgG, (b) IgG1, and (c) IgG2a subclass titers at one week after the last immunization. (d) Concentration of fentanyl in serum at 30 min post-drug challenge. (e) Linear regression of fentanyl-specific IgG titers vs. serum fentanyl concentration. (f) Concentration of fentanyl in the brain at 30 min post-drug challenge. Data are mean ± SEM. Sample size: n = 5 per group. Statistical analysis conducted by one-way ANOVA with Tukey’s multiple comparisons post hoc test. Symbols: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 compared to control, or brackets to indicate differences between groups.
