Fig. 3: Immunological correlates of protection in NHP.
Graphs represent logistic regression models with immune parameters as predictors of survival after EBOV Kikwit challenge (100 pfu, IM) in cynomolgus monkeys for: a EBOV GP-binding antibody levels (ELISA, red), and b EBOV neutralizing antibodies (psVNA, brown). c EBOV GP-reactive T cells (IFN-γ ELISpot, purple). Individual immune response levels are identified with open circles and the associated survival as a binary variable with survival as 1 (top) and nonsurvival as 0 (bottom). Assay LODs are indicated by dotted lines. d ROC curves for sensitivity and specificity of GP-binding antibody levels (red), EBOV neutralizing antibodies (brown) and EBOV GP-reactive T cells (purple) in predicting NHP survival after challenge. ROC AUC are indicated in the panel. AUC area under the curve, ELISA enzyme-linked immunosorbent assay, IFN-γ ELISpot interferon-gamma enzyme-linked immunospot, EU ELISA units, IC50 half-maximal inhibitory concentration, LOD limit of detection, psVNA pseudovirus neutralization assay, SFU spot-forming units.
