Fig. 5: Active immunisation with anti-CgoX mAb D3 epitope peptide.
From: Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase
a Competition ELISA. Binding of anti-CgoX mAb D3 to rCgoX coated on ELISA MaxiSorp plate was competed for with CgoX-D3-BSA conjugate. Binding was detected with anti-mIgG-HRP and compared to control sample (BSA). b Anti-CgoX-BSA IgG titer. Sera of two CgoX-D3-BSA immunised mice were collected at day 68 and analysed together with preimmune serum for anti-CgoX IgGs by ELISA. c Survival of mice challenged with S. aureus upon immunisation with CgoX-D3 epitope peptide conjugated with BSA. BALB/c mice (n = 11) immunised with CgoX-D3-BSA or the carrier protein BSA (black) as control group (n = 10), were infected i.p. with 3.3 × 107 cfu S. aureus USA300 mixed with 5% mucin from porcine stomach. Significance was calculated by Log-rank (Mantel-Cox) test.
