Fig. 2: Animal protection conferred by immunization of mice with rAd5-YFV or rAd5-LcrV vaccines. | npj Vaccines

Fig. 2: Animal protection conferred by immunization of mice with rAd5-YFV or rAd5-LcrV vaccines.

From: A new generation needle- and adjuvant-free trivalent plague vaccine utilizing adenovirus-5 nanoparticle platform

Fig. 2

Mice (n = 5–7/group) were immunized (i.n.) twice 21 days apart with either 1.2 × 1010 v.p. of rAd5-YFV or rAd5-LcrV vaccines, with mice receiving PBS served as controls (a). After 24 days post second immunization, animals were challenged with 100 LD50 of Y. pestis CO92-lux (b) by either i.n. route (c) or s.c. route (d) or by Y. pestis CO92 F1-negative strain via the i.n. route (e). The percent of animal survival was then plotted. P values were calculated using Kaplan–Meier analysis with log-rank (Mantel–Cox) test. Asterisks represent comparison to the control group. *P < 0.05, **P < 0.01, ***P < 0.001. Two biological replicates were performed, and data plotted.

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