Fig. 3: Pre-challenge immune response specific to lysate of M. paratuberculosis.
From: A single dose polyanhydride-based nanovaccine against paratuberculosis infection
C57BL/6 mice (n = 5) were immunized with various vaccine groups and 6 WPV, five mice from each group were euthanized. Spleens were harvested; lymphocytes were isolated and stimulated with the M. paratuberculosis lysate for 24 h. Cells were then stained for CD4+ (a) and CD8+ (b) cell surface markers and intracellular cytokines. The total percentage of T cells secreting particular cytokines are indicated below each pie chart (denoted by T = number). The error bars show the standard error of the mean for five individually analyzed mice. * indicates p < 0.05; ** indicates p < 0.001. * denotes comparison with PBS while # denotes comparison with Mycopar®. Results were expressed as the increase in the percentage of the cells with positive staining relative to that of an unstimulated sample stained with the same antibody.
