Table 1 50% paralytic dose (PD50) concentration (log10 CCID50) for various virus strains generated by molecular cloning to contain key mutations identified in the EES.

From: Assessment of genetic changes and neurovirulence of shed Sabin and novel type 2 oral polio vaccine viruses

Strain

PD50a

Sabin-2

5.9 (5.5–6.2)

Sabin-2/A481G

1.8 (1.4–2.1)

Sabin-2/U398C

4.8 (4.2–5.3)

Sabin-2/U398C/A481G

1.9 (1.4–2.3)

Sabin-2/VP1-I143V

3.5 (2.9–4.0)

Sabin-2/A481G/VP1-I143V

1.2 (0.8–1.6)

Sabin-2/U398C/A481G/VP1-I143V

1.2 (0.9–1.5)

nOPV2-c1

>8.4 (0/8)b

nOPV2-c1/123C

>6.5 (2/8)b

nOPV2-c1/VP1-I143V

7.8 (7.4–8.3)

nOPV2-c1/U123C/U459C/VP1-I143V

4.3 (3.8–4.7)

nOPV2-c2

6.3 (5.8–6.8)

nOPV2-c2/U398C

4.0 (3.3–4.6)

nOPV2-c2/VP1-I143V

3.9 (3.3–4.6)

nOPV2-c2/ U398C/VP1-I143V

2.4 (1.8–3.0)

  1. aVirulence of viruses was determined in Tg66 mice using intraspinal inoculation (see Supplementary Material for more information on Tg66 mice).
  2. bProportion of mice paralysed at highest dose.
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