Fig. 7: Summary of attenuating E protein mutations in JEV vaccine strains.

The three attenuated vaccine strains were empirically derived which led to distinct combinations of genotypes and phenotypes (A). It is apparent that genetic diversity doesn’t correlate with attenuation in mice. It is proposed that changes to the E protein of SA14-2-8 (shown in green) and SA14-14-2 (shown in blue) are responsible for differences in mouse virulence (B).