Fig. 1: Development of deimmunized flagellins.
From: Deimmunization of flagellin for repeated administration as a vaccine adjuvant
a Schematic view of deimmunized FlaB variants. Wild type Vibrio vulnificus FlaB consists of conserved N- and C-terminal domains required for flagellum biogenesis. dFlaB and FlaBΔD2D3 were deimmunized by deleting potential B-cell epitopes in ND2, D3, and CD2, where variability among different bacterial genus and species. b BepiPred-2.0 predicted ND2 to CD2 domains being most immunogenic. c The Parker hydrophilicity predicted that the most of ND2 domain may also be immunogenic. d In silico alanine scanning by FoldX additionally identified residues in little interactions with other regions (near zero). Based on these three metrics, we selected a linear region of 19 amino acids (175 SYQAEEGKDKNWNVAAGDN 193; shaded in blue) for deimmunization. The red box in ND1 represents TLR5 binding motif while blue box in CD0 represents NLRC4 binding motif, respectively. The 6H represented 6xHis-Tag for purification of recombinant dFlaB and FlaBΔD2D3.
