Fig. 6: The dFlaB-adjuvant induced better protection than that of wild type FlaB in a H1N1 influenza lethal challenge model.
From: Deimmunization of flagellin for repeated administration as a vaccine adjuvant
Groups of mice (n = 9–10) were intranasally immunized with PBS, FlaB, dFlaB, H1N1 A/Brisbane/59/07 split vaccine (sH1N1), sH1N1 plus FlaB (sH1N1 + FlaB), or sH1N1 plus dFlaB (sH1N1 + dFlaB) three times at a two-week interval. Two weeks after the immunization, mice were intranasally challenged with a 2.4 × LD50 live A/Brisbane/59/07 influenza virus. After the challenge, survival rate and changes in body weight (a) were monitored for 2 weeks. Naive mice were used as controls for the determination of body weight change. b Plaque reduction neutralization test (PRNT) using live influenza A/Brisbane/59/07 virus. Two weeks after the last immunization, serum was collected from the retroorbital plexus. PRNT50 titer was determined as 50% neutralization of plaques based on positive control virus wells. Mann–Whitney test was used to compare two groups.
