Table 1 Bacterial strains, plasmids, and influenza virus.
From: Deimmunization of flagellin for repeated administration as a vaccine adjuvant
| Â | Description | Source |
Bacteria | Â | Â |
Escherichia coli DH5α | F − recA1 restriction negative | Laboratory collection |
E. coli ER2566 | F− λ− fhuA2 [lon] ompT lacZ::T7 gene1 gal sulA11∆(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] | New England Biolabs, Inc. |
E. coli BL 21 (DE3) | hsdS gal (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene1) | Laboratory collection |
Plasmid | Â | Â |
pCR2.1TOPO | Cloning vector; Apr; Kmr | Invitrogen |
pTYB12 | N-terminal fusion expression vector in which the N terminus of a target protein is a fused Intein-tag; Apr | New England Biolabs, Inc. |
pET30a+ | N-terminal fusion expression vector in which the N terminus of a target protein is a fused His-tag; Kmr | EMD Bioscience |
pCMM250 | 1.5-kb EcoRI-PstI fragment containing ORF of flaB cloned into pTYB12 | [28] |
pET-30a(+)::dFlaB | pET-30a(+) plasmid containing a DNA-fragment of FlaB with deletion of spanned 175–193 amino acid at NdeI-XhoI | This study |
pET-30a(+)::FlaBΔD2D3 | pET-30a(+) plasmid containing a DNA-fragment of FlaB with deletion of D2D3 region at NdeI-XhoI | This study |
| Â | Â | This study |
Influenza virus | Â | Â |
H1N1 | A/Brisbane/59/2007 |
