Fig. 6: C20 and αCTLA-4 increased neoantigen-specific CD8 and switched B cells.

On day twenty, nine mice per group were sacrificed and tumors and splenocytes were analyzed by FC using the gating strategy depicted in Supplementary Fig. 2. a Frequency of E2f8 neoantigen-specific response was measured using the specific dextramer (Dx+) gated on CD45⁺CD3⁺ CD8⁺ in live cells. b Treg population (CD25⁺Foxp3⁺) were gated on CD45⁺CD3⁺CD4⁺ in live cells and expressed as ratio with DX⁺ cells (c). d Switched B cells were identified as CD19⁺IgG⁺ cells and gated on CD45⁺CD3⁺ in live cells. Nine animals per group were utilized and each symbol represents an individual sample. Significance was determined using Mann–Whitney test *p < 0.05 **p < 0.01 ***p < 0.001 ****p < 0.0001.