Fig. 4: Control of rebound viremia following antiretroviral therapy interruption.
A Intact proviruses per million CD4+ T cells quantified via an SIV-optimized digital-droplet PCR based Intact Proviral DNA Assay (IPDA) measured at week 72 post-infection, directly before ART interruption. Treatment groups were aggregated into a single group and compared to sham controls. Solid Triangles are animals in the Ad26/MVA treatment group and open circles are animals from the Ad26/MVA + Env treatment group. Red lines show the geometric mean. Statistical significance was derived from a nonparametric Mann–Whitney U Test. B–D Plasma viral loads (in log SIVmac251 gag copies/ml plasma) for each study group 198 days following ART interruption at week 72 post-infection. Red curves indicate median values. E Number of days until rebound infection was detected after ART interruption at week 72 post-infection. Red lines indicated median values. Statistical significance was determined from a Kruskal–Wallis test with Dunn’s correction for multiple comparisons. F Plasma viral loads detected at day 198 post-ART interruption, for each treatment group and sham animals. Red lines denote median values. Statistical significance was determined from a Kruskal–Wallis test with Dunn’s correction for multiple comparisons. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.
