Fig. 3: T cell epitope mapping after DNA vaccination using electroacupuncture machine. | npj Vaccines

Fig. 3: T cell epitope mapping after DNA vaccination using electroacupuncture machine.

From: A DNA vaccine candidate delivered by an electroacupuncture machine provides protective immunity against SARS-CoV-2 infection

Fig. 3

a Two peptide matrices contained sequential peptide pools X1–X16 and nonsequential peptide pools Y1–Y12. The amino acid sequence of each peptide is shown in Supplementary Data 1. b To identify the T cell epitopes of the spike protein, splenocytes from DNA vaccine-immunized mice (100 μg pSARS2-S/40 Vpp/120 Hz/5 s) were restimulated with each peptide pool for 48 h. IFN-γ-producing cells were determined by ELISpot. Based on the number of IFN-γ-producing cells, responsive stimulatory pools are marked yellow in the peptide matrices, and candidate peptides at the intersections of the stimulatory rows and columns are indicated in orange. c To identify the immunodominant T cell epitopes of the spike protein, splenocytes from immunized mice were restimulated with each candidate peptide individually. Antigenic peptides are presented in bold in the peptide matrices. The data are presented as the mean ± SEM.

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