Fig. 2: IVT-mRNA transcription and purification. | npj Vaccines

Fig. 2: IVT-mRNA transcription and purification.

From: Towards the future exploration of mucosal mRNA vaccines against emerging viral diseases; lessons from existing next-generation mucosal vaccine strategies

Fig. 2

Crude IVT-mRNA which has been generated synthetically from a linearized DNA template using polymerases (T3/T7) often contains a mixture of the mRNA molecules with different categories of impurities. The crude mRNA is initially subjected to extraction and precipitation processes which remove some but not all the impurities, final HPLC or FPLC treatment generates pure grade mRNA molecules.

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