Fig. 6: Contribution of each individual Epigraph immunogen towards the cross-reactive antibody and T-cell responses. | npj Vaccines

Fig. 6: Contribution of each individual Epigraph immunogen towards the cross-reactive antibody and T-cell responses.

From: An epitope-optimized human H3N2 influenza vaccine induces broadly protective immunity in mice and ferrets

Fig. 6: Contribution of each individual Epigraph immunogen towards the cross-reactive antibody and T-cell responses.The alternative text for this image may have been generated using AI.

To examine the contribution of each individual Epigraph immunogen, BALB/c mice (n = 5) were immunized with 3.3 × 109 vp of HAdV-5-Epigraph 1, 2, or 3 individually. Antibody responses were measured using a HI assay against the 18 H3 representative strains for both a single immunization (a) or after boosting (b). A heat map of these HI titers was constructed to further visualize the total cross-reactive antibody response of each vaccine. c The total T-cell response after boosting was determined using an interferon IFN-γ ELISpot with peptide libraries for six H3 strains. T-cell responses are reported as spot-forming cells (SFC) per million splenocytes (n = 5; one-way ANOVA with Tukey multiple comparisons). Data are presented as the mean with standard error (SEM).

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