Fig. 9: Median average percent anti-stalk blocking results for IgG and IgA antibodies against full-length HA antigens over time.

Percent stalk competition (PSC) for each sample and antigen was calculated as: 100−(AUC with competition (mini-HA added)/AUC without competition (RSV-F negative control added) × 100) where the AUC represented the area under the curve for fluorescence intensity for two different concentrations of mini-HA (10 µg and 50 µg/mL). For each subject, PSC values were then aggregated across antigens for the respective antigen group using the mean. For each vaccine group (n = 10 each), the median AUC was determined. The error bars represent the 95% bootstrap confidence interval of the median. Dots represent individual average PSC results for each subject and time point.