Fig. 3: CD103⁺ DC and the cGAS/STING pathway contribute to heat-iMVA adjuvanticity.

STING^Gt/Gt, Batf3^−/−, or age-matched WT C57BL/6J mice were intramuscularly vaccinated on day 0 and day 14 with OVA (10 µg) + heat-iMVA (an equivalent of 10⁷ pfu). a, b On day 21, mice were euthanized, and spleens and blood were collected. splenocytes were stimulated with OVA_257–264 or OVA_323–339. The expression of IFN-γ by CD8⁺ or CD4⁺ T cells was measured by flow cytometry. c, d On day 21, OVA-specific immunoglobulin G1 (IgG1) or OVA-specific immunoglobulin G2c (IgG2c) titers in the serum were determined by ELISA. Data are represented as mean ± SEM (n = 3–5; ^*P < 0.05; Two-tailed Mann–Whitney U test). Data are representative of three independent experiments.