Fig. 1: Defining immunodominance of the CD4 T cell repertoire in BALB/c and CBA/J mice expressing diverse I-A and I-E alleles. | npj Vaccines

Fig. 1: Defining immunodominance of the CD4 T cell repertoire in BALB/c and CBA/J mice expressing diverse I-A and I-E alleles.

From: CD4 T cell epitope abundance in ferritin core potentiates responses to hemagglutinin nanoparticle vaccines

Fig. 1

Identification of immunodominant ferritin epitopes was performed by IL-2 and IFNγ cytokine ELISpot following immunization with full length HA-ferritin nanoparticles. Purified CD4 T cells were restimulated with peptides spanning the sequence of H. pylori ferritin and major HA peptides. Experiments were conducted in A, B BALB/C mice (MHC II I-Ad/Ed) and C, D CBA mice (MHC II I-Ak/Ek). The fraction of the cytokine-producing CD4 T cell response specific for ferritin (black) or HA (white) is shown for E BALB/c and F CBA/J mice, respectively. Data are shown as the mean and SD of two independent experiments of five pooled mice per group.

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