Fig. 2: Defining immunodominance of the CD4 T cell repertoire in human MHC-II haplotypes. | npj Vaccines

Fig. 2: Defining immunodominance of the CD4 T cell repertoire in human MHC-II haplotypes.

From: CD4 T cell epitope abundance in ferritin core potentiates responses to hemagglutinin nanoparticle vaccines

Fig. 2

Identification of immunodominant ferritin epitopes was performed by IL-2 cytokine ELISpot following immunization with full length HA-ferritin nanoparticles. Purified CD4 T cells were restimulated with peptides spanning the sequence of H. pylori ferritin and major HA peptides. Experiments were conducted in transgenic mouse models expressing A HLA-DR1 and B HLA-DR4 human MHC class II alleles. Data are shown as the mean and SEM of three independent experiments of five pooled mice per group. C Summed cytokine response by antigen specificity across murine and human MHC class II molecules used in Figs. 1 and 2. D PBMC from 36 healthy human donors with unknown history of H. pylori infection were stimulated with a peptide pool spanning the entire sequence of H. pylori ferritin and plated in IL-2 and IFNγ cytokine ELISpot. Information about human donors is presented in Table 3. Data are shown as the mean and SD of the subjects assayed.

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