Fig. 1: Characterization of the HPV39, −68 and −70 WT VLPs. | npj Vaccines

Fig. 1: Characterization of the HPV39, −68 and −70 WT VLPs.

From: Characterization of an Escherichia coli-derived triple-type chimeric vaccine against human papillomavirus types 39, 68 and 70

Fig. 1

The WT N-terminally truncated L1 proteins were subjected to reducing SDS-PAGE (a) and western blotting (b) with a wide-spectrum linear mAb 4B3. Transmission electron microscopy (TEM) images, scale bar, 100 nm (c), high-performance size-exclusion chromatography (HPSEC) profiles (d), analytical ultracentrifugation sedimentation (AUC) profiles (e), and reconstructed cryo-EM maps (f) of the WT L1-only VLPs. Cryo-EM structures of HPV39, −68 and −70 VLPs (radially colored from 240 Å to 300 Å) were determined at final resolutions of 8.57 Å, 7.44 Å and 10.67 Å, respectively.

Back to article page