Fig. 7: Contribution of AS03 adjuvant to gene expression changes.

A Volcano plots of upregulated (red) and downregulated (blue) genes in adjuvanted G1 (IIV5/AS03 booster) vs non-adjuvanted G2 (IIV5) post boost. Differences higher than 1.5-fold are highlighted and names for the top 10 genes are shown. B Volcano plots of upregulated (red) and downregulated (blue) genes in adjuvanted G1 vs placebo (G5) groups. C Gene ontology (GO) enrichment analysis of differentially expressed genes (DEGs) at day 7 after boost for the indicated comparisons. Diagram shows top 10 enriched biological processes. Gene ontologies are ranked by their significance and dot size indicates overlap on number of genes. The GO enrichment was performed with a ranked query and multiple testing analytical correction, FDR ≤ 0.05 or −log10FDR ≥ 0.3. D Venn diagram of DEGs for the comparison between G1 (IIV5/AS03 booster) versus G2 (IIV5), and G1 (IIV5/AS03 booster) versus G5 (placebo) at day 92.