Fig. 4: Protective efficacy of a multivalent ChAd:TB vaccine against M.tb (H37Rv) persisters in the BALB/c model.
A Experimental schema, pertaining to panel B. B Lung mycobacterial burden (Log10 colony-forming unit (CFU)) 4 weeks post-ABx cessation. C Diagram depicting generation of M.tb culture filtrates for assessment of mycobacterial persisters in lung homogenates. D Left: Most probable number (MPN) estimates (Log10) to assess actively replicating bacilli (conventional media, black bars) and persisters (resuscitation media, blue bars). Right: Resuscitation Index, as calculated by a ratio of persisters-to-actively replicating bacilli. E Experimental schema, pertaining to panel F. F Left: Most Probable Number (MPN) estimates to assess actively replicating bacilli (conventional media, black bars) and persisters (resuscitation media, blue bars). Right: Resuscitation Index, as calculated by a ratio of persisters-to-actively replicating bacilli. Data presented in (B, D, F) represent mean ± SEM of n = 3 mice/group. Data are representative of one independent experiment. Statistical analysis for (B, D, F) was performed using a nonparametric Kruskal–Wallis with Dunn’s multiple comparison test.
