Fig. 3: Inhibition of hemagglutination and neuraminidase activity by sera of vaccinated mice.

Hemagglutination and neuraminidase inhibition activity of serum antibodies were assayed on days 0, 28, and 49 after immunization as indicated in Fig. 2a. a Hemagglutination inhibiting titers (HAI titers) were calculated as the highest serum dilution abolishing the hemagglutination activity of H7N9-A/PR/8/34. b Neuraminidase inhibition was calculated as the 50% NA inhibitory concentration (IC50) after incubation of H7N9-A/PR/8/34 with mice sera in enzyme-linked lectin assay (ELLA). Dots represent single animals (n = 6); horizontal line represents mean per group. Y-axis starts at detection limit; all mice at detection limit had no detectable titers. ****, p < 0.0001 (non-parametric One-way ANOVA).