Fig. 4: Secretion of IFN-γ after antigen-specific re-stimulation of splenocytes. | npj Vaccines

Fig. 4: Secretion of IFN-γ after antigen-specific re-stimulation of splenocytes.

From: A protective measles virus-derived vaccine inducing long-lasting immune responses against influenza A virus H7N9

Fig. 4

IFN-γ ELISpot analysis of murine splenocytes isolated 4 d after boost immunization. a H7- or (b) N9-specific T cells were detected after co-culture of splenocytes with JAWSII dendritic cell lines transgenic for MeV-N (black columns), H7, or N9 (grey columns). a, b Untransduced cells (NC) or (c) medium (unst.) served as negative controls (white columns). c Splenocytes were stimulated with 10 μg/ml MeV bulk antigens (black columns) or ConA (striped columns) as positive controls. Presented are means per group (n = 6), error bars indicate SD. ns, not significant; *, p < 0.05; ****, p < 0.0001 (non-parametric One-way ANOVA).

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