Fig. 5: Long-term H7N9-specific cellular immunity of aged mice. | npj Vaccines

Fig. 5: Long-term H7N9-specific cellular immunity of aged mice.

From: A protective measles virus-derived vaccine inducing long-lasting immune responses against influenza A virus H7N9

Fig. 5

a–c IFN-γ ELISpot assay after antigen-specific re-stimulation of murine splenocytes isolated 2 years after boost immunization with MVvac2-H7(P) (a–c) or with MVvac2-N9(P) (d–f). Untransduced cells (a, d) or medium (b, e) served as negative controls (white columns). H7- or N9-specific T cells were detected after co-culture of splenocytes with JAWSII dendritic cell lines transgenic for MeV-N (black columns), H7, or N9 (grey colums). Untransduced cells (NC) (a, d) or medium (unst.) (b, e) served as negative controls (white columns) while stimulation with 10 μg/ml MV bulk antigens (black columns) or ConA (striped columns) (b, e) served as positive controls. Results of individual mice are shown.

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