Fig. 2: The immunosignature of human and mouse antisera revealed the immunodominant OMPs of OMV.
From: OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans
a Infant sera collected before and after the fourth dose of different vaccines were assayed as pool on a microarray containing exclusively recombinant proteins. b Pre- and post-immunization mouse sera were pooled (preimmune pool n = 80, rMenB pool n = 10, 4CMenB pool n = 54, OMV pool n = 88) and probed onto a microarray spotted with engineered E. coli GMMA. Samples are ranked according to the relative abundance of antigens in OMV25 as shown by the triangle. Each tinted block represents the averaged reactivity of the replicated spots for each serum screened, and results are expressed as MFI values. Signals were considered positive when their MFI were greater than 5000, corresponding to the MFI of control protein spots after detection with fluorescent-labeled secondary antibodies, plus ten times the standard deviation. Three arbitrary MFI thresholds were also assigned for low (5000 ≤ MFI < 15,000), medium (15,000 ≤ MFI < 30,000 MFI) and high (MFI ≥ 30,000) reactivities. Color scale of signal intensity is reported on the right of the heatmap.
