Fig. 5: Anti-PorA, -OpcA, and -PorB functional antibodies were raised by the OMV component of the 4CMenB vaccine in humans.

a–c rSBA titers obtained with pooled OMV mice sera against wild-type and knockout strains are represented by columns. Dashed lines represent the minimal threshold of functionality observed in the presence of baby rabbit complement. d The ability of PBS buffer alone or purified recombinant PorB protein to inhibit the killing of M09929 in the presence of OMV pooled sera was tested by competitive SBA and the resulting rSBA titers are illustrated as filled or striped histograms, respectively. Competitor is indicated in brackets. rSBA ≥16 are considered positive (dashed lines). e–g The ability of pooled (n = 25) infant immune sera collected from infants who received four doses of 4CMenB to kill wild-type and knockout N. meningitidis strains was tested in hSBA. Bactericidal titers are described as columns. hSBA ≥4 are considered protective (dashed line). h Competitive hSBA was performed on M09929 using PBS or PorB protein as a competitor (filled and striped bars, respectively).