Table 1 Primer Sequences.
From: GP38 as a vaccine target for Crimean-Congo hemorrhagic fever virus
Primer | Direction | Sequence | Use |
|---|---|---|---|
GSP03 | Forward | 5'-CGATCTGTTTACGCGTGCCACCATGCACATCAGCC-3' | PCR amplification of CCHFV-coM for insertion into VSV vectors. Primer contains MluI restriction site. |
GSP06 | Forward | 5'-AGATATCACGCTCGAGGCCACCATGGTTCCTCAGG-3' | PCR amplification of RVG-333 for insertion into VSV vector. Primer contains NotI restriction site. |
GSP07 | Reverse | 5'-GAAGAATCTGGCTAGCTTACAGTCTGGTCTCACCCCC-3' | PCR amplification of RVG-333 for insertion into VSV vector. Primer contains NheI restriction site. |
GSP08 | Reverse | 5'-CTCGCCGGTGATGAAGAACT-3' | CCHFV-coM sequencing primer. |
GSP09 | Forward | 5'- ACCCTGTGAGAAACCTGCTG-3' | CCHFV-coM sequencing primer. |
GSP10 | Reverse | 5'- TTGATCACGCAGTCGGTGAA-3' | CCHFV-coM sequencing primer. |
GSP11 | Forward | 5'- CCTGAAGGCCAGCATCTTCA-3' | CCHFV-coM sequencing primer. |
GSP12 | Reverse | 5'- GCAGTAGGGGCAGATGTTGT-3' | CCHFV-coM sequencing primer. |
GSP13 | Forward | 5'- GGCGATATCCTGGTGGACTG-3' | CCHFV-coM sequencing primer. |
GSP14 | Reverse | 5- CAGTGTCTGCAGTAAGGGC-3' | CCHFV-coM sequencing primer. |
GSP15 | Forward | 5'-TGCCCTTACTGCAGACACTG-3' | CCHFV-coM sequencing primer. |
GSP16 | Reverse | 5'- ATGTTTCTGGGCTCGGACAG-3' | CCHFV-coM sequencing primer. |
GSP17 | Forward | 5'- TCAACGTGCAGTCCACCTAC-3' | CCHFV-coM sequencing primer. |
GSP18 | Reverse | 5'- TCCTCCTCGCTACAGCTCTT-3' | CCHFV-coM sequencing primer. |
GSP19 | Forward | 5'- AAGAGCTGTAGCGAGGAGGA-3' | CCHFV-coM sequencing primer. |
GSP20 | Reverse | 5'-GCTAGCTTAGCCTCTGGTTCTCCG-3' | PCR amplification of CCHFV-coM for insertion into VSV-ΔG vector for surrogate challenge virus. Primer contains NheI restriction site. |
GSP21 | Reverse | 5'-GCGGCCGCTTAGCCTCTGGTTCTCCG-3' | PCR amplification of CCHFV-coM for insertion into VcoM vector for vaccine. Primer contains NotI restriction site. |
GSP42 | Reverse | 5'-CATAGTCATCTTCATTGA-3' | Sequencing primer for RVG in VSV-ΔG-coM-RVG. |
GSP49 | Forward | 5'-GCCGCCAGTGTGCTGGAATTCGCCACCATGGAGACAGACACA-3' | PCR amplification of signal sequence for GP38 chimeric gene. |
GSP53 | Reverse | 5'-tcttcaggttGTCACCAGTGGAACCTGGAACC-3' | PCR amplification of signal sequence for GP38. |
GSP54 | Forward | 5'-CACTGGTGACaacctgaagatggagatca-3' | PCR amplification of GP38 for GP38 with a signal sequence. |
GSP55 | Reverse | 5'-AACATCGTATGGATAGTCGACGGACCCGGTGCTGGCCTT-3' | PCR amplification of GP38 for GP38 with a signal sequence. |
GSP66 | Forward | 5'-TAATACGACTCACTATAGGGGGACAGCCTGATGACATTG-3' | Primer for IVT of VSV-N. |
GSP67 | Reverse | 5'-TCTGGTGCATACAAACCT-3' | Primer for IVT of VSV-N. |
GSP68 | Forward | 5'-CAAAGAATTCCGGAACGTACGGCCACCATGGAGACAGACACA-3' | PCR amplification to put the GP38 with the signal sequence into a pCAGGS plasmid. |
GSP69 | Reverse | 5'-CCGAGGATTCGGACCCGGTGCTGGCCTT-3' | PCR amplification to put the GP38 with the signal sequence into a pCAGGS plasmid. |
GSP70 | Forward | 5'-CACCGGGTCCGAATCCTCGGTTATCCCCC-3' | PCR amplification of RABV-G 51 amino acids of the ectodomain (ED51), transmembrane domain (TM) and cytoplasmic tail (CT) to make the chimeric GP38. |
GSP71 | Reverse | 5'-GAGGGAAAAAGATCTGCTAGCTTACAGTCTGGTCTCACCCCC-3' | PCR amplification of RABV-G 51 amino acids of the ectodomain (ED51), transmembrane domain (TM) and cytoplasmic tail (CT) to make the chimeric GP38. |
GSP72 | Forward | 5'-CCTCTGCCGACTTGGCACAA-3' | Primer for qPCR of VSV-N. |
GSP73 | Probe | 5'-CCGGAGGATTGACGACTAATGCACCGCCACAAGGCAG-' | Primer-probe for qPCR of VSV-N. |
GSP74 | Reverse | 5'-CCGAGCCATTCGACCACATC-3' | Primer for qPCR of VSV-N. |
GSP84 | Forward | 5'-CAAAGAATTCCGGAACGTACGATGCACATCAGCCTGATGTACGC-3' | PCR amplification of MLD to create the chimeric GP85. |
GSP85 | Reverse | 5'-TCTTCAGGTTCCGCTTGCTCCTGTTGGTGG3' | PCR amplification of MLD to create the chimeric GP85. |
GSP86 | Forward | 5'-GAGCAAGCGGAACCTGAAGATGGAGATCATCCTGA-3' | PCR amplification of chimeric GP38 to create the chimeric GP85. |
GSP87 | Forward | 5'-AATTCCGGAACGTACGGCCACCATGGAGCTGAGG-3' | PCR amplification of human furin gene to insert into pCAGGS plasmid. |
GSP88 | Reverse | 5'- AAAAAGATCTGCTAGCTTAGAGGGCGCTCTGGTC-3' | PCR amplification of human furin gene to insert into pCAGGS plasmid. |
RP591 | Forward | 5'-GGAGGTCGACTAAAGAGATCTCACATAC-3' | Sequencing of foreign gene in BNSP333 vector. |
RP592 | Reverse | 5'-TTCTTCAGCCATCTCAAGATCGGCCAGAC-3' | Sequencing of foreign gene in BNSP333 vector. |
RP1325 | Forward | 5'-GTTATGGTGCCATTAAACCGCTG-3' | Sequencing of RVG in BNSP333 vector. |
RP1327 | Reverse | 5'-TCTCCAGGATCGAGCATCTT-3' | Sequencing of RVG in BNSP333 vector. |
VP5F | Forward | 5'-GCGTGGGTCCTGGATTCTAT-3' | Sequencing of foreign gene in VSV vector |
VP9R | Reverse | 5'-ATCGAGGGAATCGGAAGAGAAT-3' | Sequencing of foreign gene in VSV vector |
