Fig. 5: BCG+1xH107e co-administration induces BCG-specific Th17 cells.
From: A protective, single-visit TB vaccination regimen by co-administration of a subunit vaccine with BCG
a–e Pre-challenge responses at 5 weeks post vaccination. a Principal component analysis (PCA) of neutrophil, Ly6C- and Ly6C+ monocyte, CD8a+-resident, CD103+-migratory, CD11b+, and Ly6C+ DC cell numbers in vaccine-draining lymph nodes among BCG (blue), BCG + CAF01 (pink) and BCG+1x H107e (red) immunized relative to non-immunized control mice (None; gray). b Number of CD8a+ resident (left) and CD11b+ (right) DCs. Box plots showing median ± IQR with whiskers signifying the range of responses. Statistically significant differences between groups determined using Kruskall–Wallis and Dunn’s multiple comparison test, n = 6. c Magnitude of TB10.4-specific IFN-γ response from splenocytes. Box plots (left) show median ± IQR with whiskers covering range of TB10.4-specific IFN-γ-release as measured by ELISA. Kruskall-Wallis and Dunn’s multiple comparison test. Right bar chart shows the frequency of TB10.4-specific IFN-γ + CD4 T cells, One-way ANOVA with Tukey’s multiple comparison test. n = 6 in both cases. d Concatenated FACS plots of CD4 T cells showing the frequency of TB10.4-specific IFN-γ + (upper) and IL-17A+ (lower) cells among BCG (blue), BCG + CAF01 (pink) and BCG+1xH107e (red) immunized mice. e Magnitude of TB10.4-specific IL-17A release from splenocytes (left). Data shown as box plots with median ± IQR and whiskers covering range of responses. Right: frequency of TB10.4-specific IL-17A + CD4 T cells. Box plots showing median ± IQR with whiskers covering range of responses. In both cases, One-way ANOVA with Tukey’s multiple comparison test, n = 6.
