Fig. 5: The SPR-dLIA method is highly reproducible across multiple chip preparations and IgG extractions. | npj Vaccines

Fig. 5: The SPR-dLIA method is highly reproducible across multiple chip preparations and IgG extractions.

From: Calibration of a serum reference standard for Group B streptococcal polysaccharide conjugate vaccine development using surface plasmon resonance

Fig. 5

Each point represents the geometric mean of the three dilution-adjusted concentrations (DAC) of the GBS IGG 2019 material run as test samples in the dLIA. The color codes represent the materials from three individual purifications that were independently quantified via SPR and appropriately diluted in ADS to use as contrived reference standards in the dLIA. Each colored dot represents the paired test samples for those runs. The Y-axis represents the log DAC in µg/mL. Each SPR repeat was run in duplicate (n = 22 for each repeat). Points are jittered horizontally to avoid overlapping. The boxes represent 50% of the data around the median. The whiskers at the top and bottom represent ±1.5 IQR (interquartile range). Data points beyond the ±1.5 IQR are represented as black dots.

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