Fig. 1: rVSV-SUDV protects against SUDV challenge in guinea pigs.
From: The rVSV-EBOV vaccine provides limited cross-protection against Sudan virus in guinea pigs
Groups of six guinea pigs were vaccinated with rVSV-SUDV at a dose of either 2 × 105 PFU or 2 × 103 PFU. Control animals (n = 6) received an equivalent volume of saline. Twenty-eight days after vaccination, animals were challenged with 1000 LD50 of GPA-SUDV. Animals were monitored for survival (a), weight change (b), and body temperature (c). The area shaded pink in (c) highlights temperatures above 39.5 °C. Blood samples were obtained from each animal on day 5 post-infection and either day 9 post-infection or at the terminal time point (T) if it occurred before day 9. Samples were assessed for levels of virus RNA via RT-qPCR, and data are presented as Log10 genome equivalents (GEQ) per milliliter for each animal, with means and standard deviations indicated (d). A single animal (†) that was vaccinated with 2 × 105 PFU rVSV-SUDV died during sampling on day 9; this animal did not exhibit signs of disease and is considered a survivor. Survival curves (a) were compared using the Log-Rank test with the Bonferroni correction for multiple comparisons; virus RNA levels (d) were compared using a two-way ANOVA with Tukey’s multiple comparison test. **p ≤ 0.01; ****p ≤ 0.0001.
