Fig. 3: Adjuvanting F1-CRM with INI-4001 plus alum preferentially increases anti-F1 IgG2a antibody concentration.
From: A lipidated TLR7/8 adjuvant enhances the efficacy of a vaccine against fentanyl in mice
Mice were vaccinated twice, IM, with 5 µg F1-CRM plus 7.68, 9, or 22.5 µg alum, 0.1, 1, or 10 µg INI-2002, 0.1, 1, or 10 µg INI-4001, the combination of INI-2002 + alum, or the combination of INI-4001 + alum as indicated. Fourteen days after the second vaccination, blood was collected, and anti-F1 IgG (a, b), IgG1 (c, d), and IgG2a (e, f) antibody concentrations were measured by ELISA. Antibody responses to vaccines adjuvanted with INI-2002 are shown on the left (a, c, e). Antibody responses to vaccines adjuvanted with INI-4001 are shown on the right (b, d, f). Statistical analysis was conducted by one-way ANOVA with Fisher’s LSD for multiple comparisons (GraphPad Prism). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; color of asterisks indicates comparison group.
