Fig. 5: The activation of TLR4 by INI-2002 is blocked by the addition of F1, CRM, or F1-CRM.
From: A lipidated TLR7/8 adjuvant enhances the efficacy of a vaccine against fentanyl in mice
HEK cells expressing human TLR4 were stimulated with F1, CRM, F1-CRM, INI-2002, or the combination of F1, CRM, or F1-CRM plus INI-2002. The order of addition of F1, CRM, or F1-CRM and INI-2002 varied when both were used as indicated in the table by either “first” or “second”. The addition of the second component occurred 1 h after the addition of the first component. INI-4001 and vehicle only were included as negative controls. Statistical analysis was conducted by one-way ANOVA with Fisher’s LSD for multiple comparisons (GraphPad Prism). **p ≤ 0.01 and ****p ≤ 0.0001. Orange asterisks and text indicate a comparison to INI-2002, and black asterisks and text indicate a comparison to vehicle only.
