Fig. 6: Addition of INI-4001 increases antibody avidity to F1.
From: A lipidated TLR7/8 adjuvant enhances the efficacy of a vaccine against fentanyl in mice
a Mouse serum samples from Fig. 3 were used to measure the dissociation rate (Kdiss) between F1 and polyclonal mouse serum for each individual serum sample using the Octet Red 96e instrument (Fortebio). Dissociation rate constants (Kdiss) were calculated by processing raw data using ForteBio HT analysis software version 11.1.3.50. All data were inspected for quality of fit to the calculated curve (R2 > 0.95), the response between 0.25–3 (nm shift), and residual value <10% of the maximum response fitted to the curve. b Average Kdiss per group from (a) was plotted against average anti-F1 IgG antibody concentration as shown in Fig. 3a. Correlation was calculated via nonlinear regression analysis where both axes are graphed on a log scale. Statistical analysis was conducted by one-way ANOVA with Fisher’s LSD for multiple comparisons (GraphPad Prism). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; color of asterisks indicates comparison group.
