Fig. 3: MD39 constructs containing alternate linkers retain alum binding properties and antigenicity profile when conjugated to pSer.
From: Optimization of an alum-anchored clinical HIV vaccine candidate
a Table of alternate linker sequences and their attributes. b MD39 constructs containing these linkers were expressed, conjugated to pSer4 peptides, and assayed for phosphates per protein by a malachite green assay. Values plotted are means ± standard deviation. c pSer-conjugated or unmodified MD39 constructs were mixed with alum, and the fraction of protein bound to alum was assessed before (“Loading”) and after incubation for 24 h in 10% mouse serum at 37 °C. Values plotted are means ± standard deviation. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test. d Antigenicity profiling of MD39-pSer4 on alum compared to MD39_his-pSer4. Shown are the area under individual binding curves normalized to the fraction of protein loaded on alum. Values plotted are means ± standard deviation. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple comparison test. ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
