Fig. 5: Glycine spacers between pSer residues do not significantly alter the physical properties of MD39-pSer.
From: Optimization of an alum-anchored clinical HIV vaccine candidate
a MD39 was conjugated to pSer linkers containing 0–3 glycine residue spacers between pSer residues, and these constructs were mixed with alum. The fraction of protein bound to alum was assessed before (“Loading”) and after incubation for 24 h in varying amounts of mouse serum at 37 °C. Values plotted are means ± standard deviation. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test. b, c Antigenicity profiling of MD39-pSer4 constructs. Shown are the area under individual binding curves. Values plotted are means ± standard deviation. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test. ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
