Fig. 1: Characteristics of the recombinant proteins.
From: Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity
a Characterization of eukaryotic-(eFlaB) or prokaryotic- (pFlaB) expressed Vibrio vulnificus FlaB by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analysis (WB) with mouse anti-pFlaB serum. b Characterization of Peptide N-glycosidase F (PNGaseF)-digested deglycosylated eFlaB by SDS-PAGE and WB analysis. c Comparison of TLR5-dependent NF-κB stimulating activity of pFlaB, eFlaB, and PNGaseF-treated eFlaB. EC50 values were analyzed by a two-tailed Student’s t-test. ****P < 0.0001; ns non-significant.
