Fig. 3: Genetic strategy used for the generation of Qβ and Qβhp VLPs. | npj Vaccines

Fig. 3: Genetic strategy used for the generation of Qβ and Qβhp VLPs.

From: Proof of concept for a single-dose Group B Streptococcus vaccine based on capsular polysaccharide conjugated to Qβ virus-like particles

Fig. 3: Genetic strategy used for the generation of Qβ and Qβhp VLPs.The alternative text for this image may have been generated using AI.

Top panel: expression of Qβ VLPs was achieved by cloning the Qβ coat protein open-reading frame under the T7 promoter; monomers self-assemble drandomly incorporating E.coli RNA. Bottom panel: for Qβhp VLPs expression, a hairpin structure was inserted immediately downstream of the stop codon of the gene encoding the Qβ coat protein; monomers self-assembled incorporating mainly Qβhp RNA.

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